Gas chromatographic analysis of conjugated linoleic acids
نویسندگان
چکیده
The aim was to determine the conjugated linoleic acids in beef with GC-FID. Perfect separation of all of the isomers cannot be achieved, though conjugated linoleic acid isomers proved to be separable from the other fatty acids that are present in beef in a significant amount related to conjugated linoleic acids. Lipid-extraction was carried out with n-hexane/i-propanol, giving 6.8 percent higher yield than Soxhlet extraction. The variance – due to different parts of the sample preparation process – was quantified. The accuracy of the whole process and the efficiency of triglyceride conversion/fatty acid methyl ester extraction were determined.
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